Art of making iron-albuminous compounds.



1T SA ATENT onruon.

FRITZ ZUCKMAYER, F ELBERFELD, GERMANY, ASSIGNOR T0 DR. WALTHER WOLFE ANDCOMPANY, GESELLSCHAFT MIT BESCHRANKTER HAFTUNG, OF ELBEREELD,

ART OF MAKING IRON-ALBUMINOUS COMPOUNDS.

GERMANY.

1,036,405, Specification of Letters Patent.

N 0 Drawing.

To all whom timely concern:

Be it known that I, FRITZ ZUCKMAYER, citizen of the German Empire,residing at Elberfeld, Kingdom of Prussia, Germany, have inventedcertain new and useful Improvements in the Art of Making Iron-Albuminous Compounds; and I'do hereby declare the following to be afull, clear, and exact description of theinvention, such as will enableothers skilled in the art to which it appertains to make and use thesame.

This invention relates. to the manufacture of iron-albuminous substancesand has particular reference to the preparation of al buminous compoundscontaining iron and phosphorus in combination with arsenic.

The object of the invention is to obtain albuminous compounds containingiron and combined with phosphorus and arsenic in such a manner asto beavailable as therapeutic agents and in a condition in which they willnot disagreeably affect the stomach and in which they will be readilydissolved in the intestines and properly assimilated by the humanorganism.

In my applications Serial Nos. 569703 and 621234, filed June 30, 1910and April 15, 1911, respectively, I have described methods consisting incausing an iron salt and metaphosphoric acid, or a salt or com-.

pound containing the metaphosphoricacid radical, to react on analbuminous substance or upon a halogen derivative of such albuminoussubstance, and the new compounds resulting from such methods. I havefound that these methods may be modified by replacing a portion of themetaphosphoric acid or salt thereof by arsenic acid or metarsenic acid,or arsenic pentoxid or other hydrates of arsenic pentoxid, or a salt ofan arsenic acid, such modified process leading to compounds in whichmore or less arsenic is bound according as the relative propor tions ofthe phosphoric and the arsenic com pound added are varied.

In preparing the new'compounds, either the simple albuminous compoundsor their products of decomposition, such as albumoses or peptones ortheir halogen derivatives may be employed as starting materials, as inthe aforesaid prior applications, the resultant new products exhibitingsimilar properties to those of the products covered advantages due toarsenic.

in the same, together with the therapeutic In particular Patented Aug.20, 1912. Application filed December 1, 1911. Serial No. 663,359.

they are insoluble in a mixture of pepsin and hydrochloric acid andsoluble in "a dilute solution of alkali such as sodium carbonate.

More in detail it is to' 'be stated thatthe products made accordingtothe present invention are all distinguished by being insoluble in adeci-normal-solution of .hydroor cold. Another property of these newproducts is that on being treated with nitric acid solution andwarming,.yellow precipitates or secretions are separated therefrom.

In order to fully disclose my invention, I will now proceed,.to describeseveral examples of the preferred manner of carrying the same intoeffect. r 3

Example 1: 5 kilograms of casein are dissolved in- 200 liters of waterby adding thereto one kilogram of sodium hydrate solution of a strengthof 38 Baum. To this solution are added 750 grams of asolution ofmetaphosphoric acid such as. is found in the trade and thereupon 25grams of arsenic acid solution is added wherebya' white precipitate isformed. There are then cent. solution of ferric chlorid (Fe Cl,) andthereupon a dilute ammonia solution is introduced until solution occurs.From this solution the new compound is precipitated by the addition ofdilute hydrochloric acid, the precipitate so obtained being subsequentlywashed, pressed and dried. The

new iron albuminous compound so obtained contains about 1.5 per cent. ofarsenic, 8 per cent. of iron, 8 per cent. of phosphoric acid anhydridand 10.2 per cent. of nitrogen.

Example ,2: 5 kilo-grams of dried white of egg are dissolved in 150liters of water and to the solution so prepared 4 liters of a 50 percent. ferric chloric solution are,

added, whereby a yellowish-brown precipitate is formed. To this mixturethere is then added an aqueous solution of 100 grams of arsenic acid andan aqueous solution of 900 grams of vitreous phosphoric acid, the;

whole bein then rendered feebly alkaline by the addition thereto ofdilute soda lye (sodium hydrate solution). Dilute hydrochloric acid isthen added to this solution chloric acid whether such solution be warmand the resultant precipitate separated by filtration, washed and dried.The new product obtained by this method contains talbumose of casein,prepared in the usual therefrom.

uni-hydrate) manner, are dissolved in 150 liters of water with the aidof dilute ammonia solution and to the solution so obtained there isadded an aqueous solution of 750 grams of vitreous phosphoric acid andan aqueous solution of 250 grams of arsenic acid. A

yellowish-white precipitate .is thereby .formed. 3 liters of a per cent.solution of ferric chlorid is now added to the whole and the mixturereduced to a state of solu tion by the addition of a sufficient quantityof dilute ammonia solution, from which solution the new product is thenthrown down by the addition of dilute hydrochloric acid, separated byfiltering, washed and dried as in the preceding examples. The newproduct contains substantially 1.3 per cent. of arsenic, 6.5 per cent.of iron, 12.6 per cent.

of-phosphoric acid anhydrid and 10 per eent. of nitrogen.

The new products obtained by the methods described for the three aboveexamples are distinguished by the following properties: They areinsoluble in a deci-normal solution'of hydrochloric acid'at ordinarytemperatures and on heating, but soluble in dilute solutions of alkalisand alkali-carbonates. They are therefore, insoluble in the) stomach,whereas they readily go into solution in the intestines so as to bethere assimilated. They are insoluble in a hydrochloric acid solution of6 Baum. Upon heating the new products together with nitric acid yellowexcretions are separated \Vhen dissolving one of these products in aone-half per cent. soda (sodisolution and adding ammo nium-sulfid [(NHQS1 a green'coloration without precipitate is obtained. Phosphoric acidcan be detected in the new com- ."pounds only after decomposition orincin- 50- erating or burning them.

. The I new compounds possess valuable therapeutic properties and serveas reme- .dies in anemic and chlorotic' disorders of all kinds; also incases of nervous debility and hystrics. It ihay "be employed withadvantage for convalescents, and in cases of persistent and obstinateskin-diseases. The

close is governed by the content of arsenic and in general is to befixed as five onethou-. sandt-hs of a gram 0.005 gr.) of arsenic per dayfor adults, which dose may in certain "cases be doubled. For children aquantity containing a smaller amount. of arsenic is to be prescribed.

.The order in which the reagents are effected a solution is formed andno precipitate occurs on adding a dilute alkali in sufli cient quantityto produce feeble alkaline reaction.

Instead of arsenic acid or arsenic pentoxid employed in theexamples'given, other hydrates of the pentoxid or a salt of the variousarsenic acids may be employed for the purposes of this invention.

Claims:

1. The process which consists in causing an iron compound and a compoundcon taining the metaphosphoric acid radical and an arsenic compound toreact on an'albuminous compound.

2. The process which consists in causing an iron compound and a compoundcontaining the meta-phosphoric acid radical and an arsenic compound toreact on an albuminous compound in such relative proportions that asolution is formed on rendering the resultant mixture feebly alkaline.

3. The process which consists in causing an iron compound and a compoundcontaining the metaphosphoric acid radical and'a compound containing theradical of arsenic acid, to react on an albuminous compound. 4.. Theprocess which consists in causing an iron compound and a compoundcontaining the metaphosphoric acid radical and a compound containing theradical of arsenic acid, to react on an albuminous compound in suchrelative proportions that a solution is formed on rendering theresultant mixture feebly alkaline.

5. The process which consists in causing an iron salt,a compoundcontaining the metaphosphoric acid radical and arsenic acid to react onan albuminous compound.

6. The process which consists in causing an iron salt, a compoundcontaining the metaphosphoric acid radical and arsenic acid to react onasolution of an albuminous compound.

7. The process which consists in causing an iron salt, metaphosphoricacid and'arsenic acid to react on an albuminous compound.

'8. The process which consists in causing an iron salt, a compoundcontaining the met-aphos phoric acid radical and arsenic acid to reacton an albuminous compound,

'and'then adding dilute ammonia until solution occurs.

9. As a new composition of matter, an iron albuminous compoundcontaining phosphorus and arsenic and having the following properties:it is insoluble n a decinor I mal solution of hydrochloric acid atordinary temperatures and on. heating, but soluonly after decomposing,incinerating or ble in dilute solutions of alkalis and alkaline vburningthe compound. 10 carbonates; on heating the new products In testimonywhereof I hereunto afiix my with nitric acid yellow excretions aresepasignature in the presence of two Witnesses.

rated therefrom; when dissolving in a one- FRITZ ZUCKMAYER. [1 8.] halfper cent. sodium hydrate solution and Witnesses: 4 adding ammoniumsulfid a green coloration. HELEN NUTER;

is obtained; the phosphorus can be detected ALBERT N FER.

